Collingwood has all but ruled gun midfielder Adam Treloar out of June 11's AFL return match against Richmond.
Treloar suffered a minor calf strain at the Magpies' first small group training session last Monday and hasn't been involved in full training this week.
READ: Fixture for rounds two to five revealed
"We wouldn't be anticipating that 'Ads' would be available to play in round two," coach Nathan Buckley told reporters on Wednesday.
Buckley emphasised Treloar's injury was "really low grade" but he was unlikely to reach the training intensity required to compete for selection in a fortnight.
"I don't think that's (Treloar missing round two is) any real surprise if he hasn't been able to complete a training session to this point and we're really only two weeks away from being asked to play," Buckley said.
"As we've always said, we'll pick our hardest and fittest, most connected 22 or 24 depending on where that (interchange numbers) settles for the next game, and Ads will still have to do a bit of work to prove he's in that mix."
Brody Mihocek (foot soreness) will train fully on Friday while Travis Varcoe and Lynden Dunn are set to join main training.
Tom Langdon (knee) remains on an indefinite break.
Irish pair Mark Keane and Anton Tohill are in quarantine until June 6 after returning from overseas.