Tyson Goldsack and Nathan Brown have been brought into the 27-man squad after sitting out the first two rounds of the NAB Cup.
Goldsack underwent ankle surgery last November after originally sustaining the injury against St Kilda in round 19 last season, while Brown is back after back tightness ruled him out of the club's opening fixtures against the Western Bulldogs and Essendon.
Several of Collingwood's biggest names will be watching on the from the grandstands, including Dane Swan, Scott Pendlebury, Travis Cloke, Ben Reid and Alan Toovey.
Reid appeared to hurt his right knee during the third quarter of last week's win over West Coast, but Director of Football Geoff Walsh said that, while he will not play this week, the injury should not cause any interruption to Reid's season.
The return of Goldsack and Brown and inclusion of Ben Kennedy means that, should all three make the final 24-man team, Collingwood will have used 35 of the 47 players on its entire playing list.
The high number of players rotated through the team has exposed the club's depth to the intensity of senior football, while last Sunday's victory against West Coast at Patersons Stadium provided the younger members of the team the invaluable experience of winning on the road against a high quality opponent.
Meanwhile, Brisbane will be led out by Jonathan Brown for the first time this season. Brown, who did not play in either of the Lions first two NAB Cup matches, will take the reigns alongside new co-captain Jed Adcock.
Collingwood Squad - NAB Cup Round Three
2. Jordan Russell
3. Brent Macaffer
5. Nick Maxwell
6. Tyson Goldsack
8. Harry O'Brien
9. Marty Clarke
11. Jarryd Blair
14. Clinton Young
15. Jarrod Witts
16. Nathan Brown
17. Dayne Beams
18. Darren Jolly
21. Quinten Lynch
22. Steele Sidebottom
24. Josh Thomas
26. Ben Johnson
27. Ben Kennedy
28. Ben Sinclair
37. Kyle Martin*
38. Peter Yagmoor*
39. Heath Shaw
40. Paul Seedsman
41. Sam Dwyer*
43. Adam Oxley*
45. Jack Frost*
46. Marley Williams
48. Caolan Mooney*
*Denotes rookies
IN: Brent Macaffer, Tyson Goldsack, Clinton Young, Nathan Brown, Dayne Beams, Darren Jolly, Ben Johnson, Ben Kennedy, Peter Yagmoor, Marley Williams.
OUT: Scott Pendlebury, Jamie Elliott, Ben Reid, Ben Hudson, Travis Cloke, Alan Toovey, Dane Swan.
Brisbane Squad
1. Sam Docherty
3. Brent Moloney
5. Billy Longer
6. Josh Green
7. Jed Adcock
8. Rohan Bewick
9. Ash McGrath
10. Daniel Rich
11. Pearce Hanley
12. Stefan Martin
15. Dayne Zorko
16. Jonathan Brown
17. Claye Beams
18. Todd Banfield
21. Daniel Merrett
22. Marco Paparone
23. Matthew Leuenberger
24. Joel Patfull
26. Elliott Yeo
28. Patrick Karnezis
29. Andrew Raines
31. James Polkinghorne
38. Tom Rockliff
41. Mitchell Golby
42. Justin Clarke*
44. Aaron Cornelius
46. Sam Michael*
*Denotes rookie
Note: For fans wishing to travel to and from Etihad Stadium via train, Metro Trains has announced that there will be no trains between Clifton Hill and Parliament Stations on the Hurstbridge and South Morang lines this weekend due to the replacement of a rail bridge across Victoria Street near North Richmond Station. Train replacement buses will be running between Clifton Hill and Parliament Stations all weekend. For further information, please click here.
